By Michael J. Soares, Joan S. Hunt
A suite of state of the art laboratory options for the examine of the trophoblast and placental biology. The innovations provided variety from experimental animal types, to animal and human placental organ and mobilephone tradition structures, to morphological, biochemical, and molecular recommendations for assessing trophoblast/placental development, differentiation and serve as. quantity 2 offers effortlessly reproducible protocols for learning trophoblast invasion, maternal immune phone responses to being pregnant, and the hormone generating functions of the placenta. There also are ways to examine the barrier functionality of the placenta, its function in regulating the delivery of meals and wastes, and the diversifications of trophoblast cells and the placenta to sickness. Highlights comprise suggestions to investigate trophoblast cellphone interactions with the uterine vasculature and immune cells, and the endocrine features of the placenta. A better half first quantity concentrates on tools for examine embryo-uterine implantation, trophoblast mobile improvement, and the association and molecular characterization of the placenta.
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Extra info for Placenta And Trophoblast: Methods And Protocols
4. Keep the face of the mouse up and locate the vagina. 5. 9% NaCl) and insert the tip superficially, but not deeply, into the vagina. 6. Gently squeeze the bulb of the dropper to release saline inside the vagina. Slowly release the pressure on the bulb to aspirate the vaginal lavage inside the dropper. 2. Identification of the Stage of the Estrous Cycle 1. Place a drop of aspirated vaginal fluid on a glass slide. 2. Unstained material is observed under a light microscope with 10× and 40× objective lenses to identify the stage of the estrous cycle.
No. 08-757-100B). 37 mL lactic acid (sodium salt), and 300 mg bovine serum albumin. 8. BD brand disposable 3-mL syringe (Fisher Scientific, cat. no. 14-829-14B). 9. Hamilton 31-gauge steel needle (Fisher Scientific, cat. no. 14-815-619). 10. 27-gauge BD PrecisionGlide sterile disposable needle (Fisher Scientific, cat. no. 14-826-48). 2. DETECTION OF EARLY IMPLANTATION SITES BY INTRAVENOUS DYE INJECTION 1. Anesthesia (Avertin). 2. Paper towels. 3. , Franklin Lakes, NJ; cat. no. 309623) 4. ). 5. 9% sodium chloride, Baxter Healthcare Corporation, cat.
The oviduct is flushed to recover preimplantation embryos. 3. Cut the other end of the horn just below the utero–tubal junction and keep the uterine horn in a clean moistened tissue paper to absorb blood. 4. Hold one of the uterine horns at the utero–tubal junction and insert the tip of a 27gauge needle, with a 3-mL plastic syringe filled with Whitten’s medium attached, inside the uterine lumen (Fig. 4). 02_Deb_7_34_F 20 8/29/05, 11:15 AM Mouse Implantation Methodologies 21 Fig. 3. Schematic representation to show excision of uterine horns.