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By Christopher Earland

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Weigh the Kieselgel G (10 g) into a dry 100 ml conical flask. Add distilled water (20 ml) all at once, stopper and shake thoroughly for 75 s. Pour the slurry into the spreader and draw smoothly and continuously over the clean glass plates or slides in the spreading jig taking about 5 s. Allow the plates to remain undisturbed in the jig for 5-10 min to set. Then, handling only by the edges, place in the plate holder and allow to air-dry overnight. If 'activated' plates of greater absorptive properties are required, heat the coated plates in an oven at 160-170°C for 1 h, cool and store in a desiccator over phosphorus pentoxide.

The delivery tube should be adjusted during the course of the distillation so that it is always in the correct position. Neutralise the acid in the distillation flask by adding sodium 24 hydroxide solution (20 g sodium hydroxide + 50 ml water) through the funnel provided, taking care that no ammonia can escape. Bring the flask to the boil using an electric heating mantle. This should ensure even heating and prevent breakage of the flask through thermal shock. Continue the distillation for 30-45 min, and then wash the condenser down with distilled water.

Techniques in Protein Chemistry. Elsevier, Amsterdam, 36 (1967) 35 12 Chromatography on ion-exchange paper APPARATUS Shandon Unikit No. 1. R. sodium chloride, sodium acetate, acetic acid, n-butanol, phenol, ninhydrin locating reagent (see page 31) range of amino acids, wool hydrolysate (see page 31). DISCUSSION In Whatman ion-exchange papers DE81 and P81, the primary hydroxyl group attached to carbon atom 6 of the cellulose molecule has been modified. DE81 is diethylaminoethyl cellulose (DEAE cellulose) and P81 is cellulose phosphate.

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