By Park K.-H., Robyt J.F., Choi Y.-D.
Seoul nationwide collage, Suwon, Korea. learn on enzymology and carbohydrate engineering. according to symposia held in Suwon, Korea in August, 1994, and September, 1995, subsidized via the study middle for brand spanking new BioMaterials in Agriculture. 29 participants, 7 united states
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Extra info for Enzymes for Carbohydrate Engineering
The DNA f r a g m e n t s were sequenced in both strands. The BLTA gene coded for a 55 kD protein of 483 amino acids. , 1986). The protein was localized in both periplasmic and cytoplasmic spaces. 5 kD. There was no signal sequence like sequence found at the amino t e r m i n a l of the gene. The protein was found only in cytoplasm of E. coli and the mother strain. , 1993) and four highly conserved regions were found in Table 3. Conserved regions III and IV are considered to include active sites of amylases.
Seymour, F. , Knapp, R. , Chen, E. C. , Jeanes, A. and S. H. Bishop. 1979e. Structural analysis of dextrans containing 4-O-a-D- glucosylated a -D-glucopyranosyl residues at the branch points, by use o! 13C-nuclear magnetic resonance spectroscopy and gas-chromatography-mass spectrometry, Carbohydr. Res. 7 5 : 2 7 5 - 2 9 4 . Seymour, F. R. and R. D. Knapp. 1980a. Structural analysis of a-D-glucans by 13C-nuclear magnetic resonance, spin-lattice relaxation studies, Carbohydr. Res. 81 : 67-103. Seymour, F.
Thus, by disproportionation, various kinds of oligosaccharides may be formed. Radioactive G2 and G3 were formed at the initial stage of the reaction. This implies that CGTase can also hydrolyze radioactive fraction Bs from the reducing end. By the combination of the results described above, we proposed an enzyme model of CGTase active site as in Figure 7. From the model, diglucosyl-CD will be not reacted with CGTase depending on the structure. Figure 7. Active site model of CGTase. A c t i o n o f CGTase on the m i x t u r e of (G1)2-~-CD and glucose The mixture of glucose and (G1)2-c~-CD was reacted with CGTase, and the reaction mixture injected to obtain HPLC profiles as in Figure 8.