By Professor Dr. Y. P. S. Bajaj (eds.)
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8% agar (Novak and Juvova 1982). Chee and Pool (1985) found that the vitamins thiamine and 16 P. L. Monette inositol were necessary and that nicotinic acid and pyridoxine were beneficial for shoot multiplication. The cytokinin benzyladenine (BA) elicits the greatest shoot multiplication and has generally been used at a concentration of 5 or 10 JlM (Harris and Stevenson 1982; Chee and Pool 1985). Kinetin and N-dimethylallyladenine (2iP) were not as suitable as BA (Novak and Juvova 1982; Reisch 1986).
1985) Dual culture with powdery mildew Screening of cvs. for resistance to powdery mildew Screening of cvs. for resistance to Agrobacterium; bypassing protoplast technology for gene transfers Selection of NaCItolerant rootstocks Heintz et al. (1985) Axillary bud cultures Leaf discs Shoot tip cultures Agrobacterium tumefaciens and A. rhizogenes gall formation in vitro V. rupestris Scheele Anther-derived callus and cell suspensions Ovules of seedless grapes NaCI tolerance Pinot clone 113 Barlass and Skene (1983) In vitro plantlets from axillary buds Vanessa Seedless, Remaily Seedless V.
Studies with protoplasts and isolated vacuoles. ). Vitis 22:9-14 Campbell RA, Durzan DJ (1975) Induction of multiple buds and needles in tissue cultures of Picea glauca. Can J Bot 53:1652-1657 Chee R, Pool RM (1982) The effects of growth substances and photoperiod on the development of shoot apices of Vilis cultured in vitro. Sci Hortic 16:17-27 Chee R, Pool RM (1983) In vitro vegetative propagation of Vitis: Application of previously defined culture conditions to a selection of genotypes. Vitis 22:363-374 Chee R, Pool RM (1985) In vitro propagation of Vitis: The effects of organic substances on shoot multiplication.